Whey protein hydrolysates enhance water absorption in the perfused small intestine of anesthetized rats |
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Authors: | Kentaro Ito Makoto Yamaguchi Teruyuki Noma Taketo Yamaji Hiroyuki Itoh Munehiro Oda |
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Affiliation: | 1. Food Science Research Laboratories, R&2. D Division, Meiji Co., Ltd., Odawara, Japan;3. College of Bioresource Sciences, Nihon University, Fujisawa, Japan |
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Abstract: | We evaluated the effect of whey protein hydrolysates (WPH) on the water absorption rate in the small intestine using a rat small intestine perfusion model. The rate was significantly higher with 5 g/L WPH than with 5 g/L soy protein hydrolysates or physiological saline (p?0.05). WPH dose-dependently increased the water absorption rate in the range of 1.25–10.0 g/L. WPH showed a significantly higher rate than an amino acid mixture whose composition was equal to that of WPH (p?0.05). The addition of 4-aminomethylbenzoic acid, an inhibitor of PepT1, significantly suppressed WPH’s enhancement of water absorption (p?0.05). The rate of water absorption was significantly correlated with that of peptides/amino acids absorption in WPH (r?=?0.82, p?0.01). These data suggest that WPH have a high water absorption-promoting effect, to which PepT1 contributes. |
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Keywords: | whey protein hydrolysates water absorption amino acid mixture PepT1 |
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