Purification of mitochondria and mitochondrial nucleic acids from embryogenic suspension cultures of a gymnosperm,Larix x leptoeuropaea |
| |
Authors: | Raman Kumar Marie-Anne Lelu Ian Small |
| |
Institution: | (1) Station de Génétique et d'Amélioration des Plantes, INRA, Route de St-Cyr, 78026 Versailles Cedex, France;(2) Station d'Amélioration des Arbres Forestiers, INRA, Ardon, F-45160 Olivet, France |
| |
Abstract: | After a number of attempts to isolate mitochondria from different conifer tissues, embryogenic suspension cultures of hybrid larch (Larix x leptoeuropaea) were developed which enabled the purification of mitochondria using slight modifications to standard techniques. The mitochondrial purity was verified by analysis of the mitochondrial RNA, DNA and proteins. The larch mitochondrial genome size is surprisingly large (> 1000 kbp) and the polypeptide pattern differs greatly from those of wheat or potato mitochondria, suggesting that valuable evolutionary insights will be gained from comparisons between gymnosperm and angiosperm mitochondria. The ease with which embryogenic conifer suspensions can be initiated and used for mitochondrial purification implies that they will be the material of choice for future studies of this type.Abbreviations PCR
polymerase chain reaction
- mtDNA
mitochondrial DNA
- 2,4-D
2,4-dichlorophenoxyacetic acid
- BA
N6-benzyladenine
- Tris
tris(hydroxymethyl)aminomethane
- EDTA
ethylenediaminetetraacetic acid
- BSA
bovine serum albumin
- EGTA
ethylene glycol-bis(ß-aminoethyl ether) N,N,N ,N -tetraacetic acid
- SDS
sodium dodecyl sulphate
- DEPC
diethyl pyrocarbonate
- PAGE
polyacrylamide gel electrophoresis
- IEF
iso-electric focusing |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|