| Abstract: | Peganum harmala callus culture growing on a fully defined mediumcontaining the auxin 2,4-D was found to form roots and secondarymetabolites when the auxin content of the medium was reduced.The secondary metabolites included alkaloids, lignin, and ared pigment. The interrelationships between these phenomenaare discussed. Alkaloids appeared in the rootlets and the dynamicsof their production were followed. Suitable sources of N, Fe,C, and energy were essential for alkaloid synthesis; other nutrientswere needed for its maximal expression. Phosphate-free mediastimulated secondary metabolite formation while limiting growthand morphogenesis. Light raised the content of the dihydro-ß-carbolinealkaloids relative to the fully aromatic derivatives but didnot alter the total accumulation of alkaloids. |
