聚合酶链反应用于脊髓灰质炎病毒的诊断和定型

IDENTIFICATION OF POLlOVlRUS TYPES USING POLYMERASE CHAIN REACTION

This paper reports the identification of three types of poliovirus and Sabin-related strain by polymerase chain reaction ( PCR ) . Four pairs of synthetic oligonucleotide primers are used. The pair primer map 7 and map 14 which is homologous to all enterovirus can produce 115bp nucleic fragment by PCR amplification. Other three pairs ( Sabin I-1 and Sabin I-2, Sabin II-1 and Sabin II-2, Sabin III-1 and Sabin III-2) which are specific to Sabin I, Sabin I, and SabinI can produce 97bp, 7lbp and 44bp nucleic fragment by PCR respectively. The method is sensitive and TCID 50/ml 101.5 titre of virus can be detected by PAGE after amplification. Six isolates were examined by PCR using three pairs of specific primers. Of four strain which were isolated from Liaoning and Zhejiang Province, three were positive to Sabin I and one was positive to Sabin I. The result is accordance with neutralization and IgM test. Another two strains isolated from the poliomyelitis epidemic in Shandong Province were negative when the Sabin primers were used.