The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish |
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Authors: | Donald Joshua Cameron Faydim Rassamdana Peony Tam Kathleen Dang Carolina Yanez Saman Ghaemmaghami Mahsa Iranpour Dehkordi |
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Affiliation: | 1.College of Optometry, Western University of Health Sciences;2.Molecular Neurobiology Group, Western University of Health Sciences;3.Graduate College of Biomedical Sciences, Western University of Health Sciences |
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Abstract: | Zebrafish are a proven model for vision research, however many of the earlier methods generally focused on larval fish or demonstrated a simple response. More recently adult visual behavior in zebrafish has become of interest, but methods to measure specific responses are new coming. To address this gap, we set out to develop a methodology to repeatedly and accurately utilize the optokinetic response (OKR) to measure visual acuity in adult zebrafish. Here we show that the adult zebrafish''s visual acuity can be measured, including both binocular and monocular acuities. Because the fish is not harmed during the procedure, the visual acuity can be measured and compared over short or long periods of time. The visual acuity measurements described here can also be done quickly allowing for high throughput and for additional visual procedures if desired. This type of analysis is conducive to drug intervention studies or investigations of disease progression. |
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Keywords: | Neuroscience Issue 80 Zebrafish Eye Movements Visual Acuity optokinetic behavior adult |
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