Analogues of tentoxin: Tools for mechanistic investigations |
| |
Authors: | Florine Cavelier Christine Enjalbal Jérome Santolini Francis Haraux Claude Sigalat Jean Verducci and François André |
| |
Institution: | (1) LAPP, UMR 5810 CNRS, Universités Montpellier I et II, UM II, F-34095 Montpellier Cedex 5, France;(2) CEA, Déptartement de Biologie Cellulaire et Moléculaire, Section de Bioénergétique, Centre d'Études de Saclay, F-91191 Gif-sur-Yvette Cedex, France |
| |
Abstract: | Tentoxincyclo-(MeAla1-Leu2-Me Phe3-Gly4] is a metabolite isolated from a phytopathogenic fungusAlternaria alternata, which induces chlorosis of many plants. This potentialnatural herbicide binds specifically to the soluble part CF1of the chloroplastic coupling factor, which is a proton ATP-synthase. Theeffect of the toxin is concentration dependent: at low concentration it is apowerful inhibitor, while at higher concentration, it stimulates the enzyme.We synthesized tentoxin and we designed new analogues in order to vary thehydrophobicity on the side chain and to study the structure activityrelationships. Comparative activities suggest that it is possible toseparate inhibitory and activating effects using tentoxin analogues, showingsome evidence for the existence of two binding sites with different affinityconstant. |
| |
Keywords: | Cyclopeptide Tentoxin F0F1H+-ATPase Herbicide |
本文献已被 SpringerLink 等数据库收录! |
|