首页 | 本学科首页   官方微博 | 高级检索  
     


Heterologously expressed protein phosphatase calcineurin downregulates plant plasma membrane H+-ATPase activity at the post-translational level
Authors:Marín-Manzano Mari Carmen  Rodríguez-Rosales Maria Pilar  Belver Andrés  Donaire Juan Pedro  Venema Kees
Affiliation:Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, Apartado 419, 18008 Granada, Spain.
Abstract:To investigate the effects of calcineurin expression on cellular ion homeostasis in plants, we have obtained a transgenic cell culture of tomato, expressing constitutively activated yeast calcineurin. Transgenic cells exhibited reduced growth rates and proton extrusion activity in vivo. We show that reduction of plasma membrane H+-ATPase activity by expression of calcineurin is the basis for the observed phenotypes. Transgenic calli and cell suspensions displayed also increased salt tolerance and contained slightly higher Ca2+ and K+ levels. This demonstrates that calcineurin can modulate ion homeostasis in plants as it does in yeast by affecting the activity of primary ion transporters.
Keywords:
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号