雌、孕激素对贝美格致痫大鼠大脑皮层、海马Glu和GABA免疫反应细胞的影响

采用免疫细胞化学双PAP法，观察雌二醇（E2）、孕酮（P）对贝美格（Bemegride,Be）腹腔致痫大鼠顶叶大脑皮层、海马CA1、CA3区Glu和GABA免疫反应细胞的影响。图像分析结果显示：Be致痫组皮层、海马Glu免疫反应平均阳性细胞数及光密度较正常组明显增加（P＜0.01）；CABA细胞数及光密度减少（P＜0.01）。给予E2后，Be致痫大鼠大脑皮层、海马Glu阳性细胞数目增多，光密度增高（P＜0.01），GABA阳性细胞数目减少、光密度降低（P＜0.05，P＜0.01）而给予P后，致痫组GABA阳性细胞数目增多、光密度增高（P＜0.01），Glu阳性细胞数目减少、光密度减低（P＜0.01）。提示雌、孕激素的致痫、抗痫作用与其调节脑内GABA和Glu系统的兴奋性有关。

EFFECTS OF ESTROGEN, PROGESTIN OF GABA AND GLU IMMUNOREACTIVE CELLS OF CEREBRAL CORTEX AND HIPPOCAMPUS IN SEIZURE RATS INDUCED BY BEMEGRIDE

In present study, to elucidate further mechanisms of estrogen and progestin, we observed the effects of estradiol(E 2) and progesterone(P) on GABA and Glu immunoreactive (IR) cells of cerebral cortex and hippocampus in convulsive rats after acute Bemegride(Be) administration (25mg/kg, intraperitoneally) by means of immunocytochemical method. The experimental results demonstrated that the number and light density of GABA IR cells of cerebral cortex and hippocampus in convulsive rats induced by Bemegride(Be) were decreased while those of Glu IR cells increased. After E 2 administration, the number and light density of Glu IR cells were higher, and GABA IR cells were lower than those of the convulsive rats induced by Be alone. On the contrary, the number and light density of GABA IR cells were increased and Glu IR cells were decreased following administrating of P, as compared with the convulsive rats simply induced by Be. Image analysis showed significant differences about above mentioned results ( P <0 01 or P <0 05). These findings suggested that estrogen enhanced and progestin attenuated epileptic activity via regulating the excitability of GABAergic and Glutamatergic systems in brain.