Relationship of embryogenic competence in maize (Zea mays L.) leaves to mitotic activity,cell cycle and nuclear DNA content期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Relationship of embryogenic competence in maize (Zea mays L.) leaves to mitotic activity,cell cycle and nuclear DNA content

Authors:	Marie Dole?elová Jaroslav Dole?el Milan Ne?tický

Institution:	(1) Royal Botanic Gardens Sydney, Mrs Maquarie's Road, 2000 Sydney, N.S.W., Australia;(2) School of Crop Sciences, University of Sydney, 2006, N.S.W., Australia;(3) Department of Viticulture and Enology, University of California, Davis, California, USA

Abstract:	Axillary bud explants of 11 selected mature waratah clones were established in vitro on a modified Murashige & Skoog medium. Adequate proliferation of axillary shoots was achieved by optimisation of the growth regulator status of the culture medium. For the majority of clones, a three to six times rate of proliferation was achieved with 1.25 M BA and 1.0 M GA₃ without the occurrence of abnormalities. The white flowering clone did not respond favourably to the addition of GA₃ to the medium.Abbreviations BA benzyladenine - GA₃ gibberellic acid - IBA indole-3-butyric acid - LSD least significant difference - MS Murashige & Skoog medium

Keywords:	growth regulators in vitro propagation waratah
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