Treatment of Cells with Detergent Activates Caspases and Induces Apoptotic Cell Death |
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| Authors: | W Strupp G Weidinger C Scheller R Ehret H Ohnimus H Girschick P Tas E Flory M Heinkelein C Jassoy |
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| Institution: | (1) Institute for Virology and Immunobiology, University of Würzburg, Versbacher Strasse 7, 97078 Würzburg, Germany, DE;(2) Children's Hospital, University of Würzburg, 97078 Würzburg, Germany, DE;(3) Department of Anaesthesiology, University of Würzburg, 97078 Würzburg, Germany, DE;(4) Institute of Medical Radiation and Cell Research, University of Würzburg, 97078 Würzburg, Germany, DE |
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| Abstract: | Due to their amphiphilic properties, detergents readily disrupt cellular membranes and cause rapid cytolysis. In this study
we demonstrate that treatment of cells with sublytic concentrations of detergents such as Triton X-100, Nonidet P-40, n-octylglucoside
and the bile salt sodium deoxycholate induce typical signs of apoptosis including DNA fragmentation and cleavage of poly(ADP-ribose)
polymerase molecules. The detergent concentration required for apoptosis was below the critical micellar concentration. Induction
of apoptosis was not restricted to human cells but similarly occurred in a variety of other vertebrate cell lines. Unstimulated
peripheral blood mononuclear cells were susceptible to apoptosis induction by detergent suggesting that apoptosis in this
circumstance is not mediated by CD95. Cell death was not due to influx of calcium from the medium. Apoptosis was blocked and
cytolysis prevented by treatment with peptide inhibitors of caspases. These findings suggest a process of apoptosis that is
initiated upon nonspecific alterations at the cell membrane level. Physiologic correlates of this process still have to be
defined.
Received: 12 November 1999/Revised: 6 March 2000 |
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| Keywords: | : Detergent — Triton X-100 — Nonidet P-40 — n-Octylglucoside — Sodium deoxycholate — Apoptosis — Caspase — Jurkat T lymphoblast |
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