Enantiomeric resolution of amino acids by thin-layer chromatography |
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Authors: | Yoko Nagata Teruhito Iida Masaki Sakai |
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Institution: | Department of Life Science, Himeji Institute of Technology, Himeji, Hyogo 671-2201, Japan |
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Abstract: | A simple and rapid method of separating optical isomers of amino acids on a reversed-phase TLC plate, without using impregnated plates or a chiral mobile phase, is described. Amino acids derivatized with 1-fluoro-2,4-dinitrophenyl-5-
-alanine amide were spotted on a reversed phase pre-coated TLC plate. Enantiomers of glutamate and aspartate were separated most effectively with solvent consisting of 25% acetonitrile in triethylamine-phosphate buffer (50 mM, pH 5.5) (v/v). Separation of
- and
-serine was achieved with 30% of acetonitrile solvent. The enantiomers of threonine, proline and alanine were separated with 35% of acetonitrile solvent, and those of methionine, valine, phenylalanine and leucine with 40% of acetonitrile solvent. The possibility of using TLC for quantitative determination of amino acid enantiomers was shown by the quantitative recovery of
- and
-alanine from the TLC plate in the range of 0.56–4.48 nmol. |
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Keywords: | Thin-layer chromatography Amino acid Enantiomer Resolution Analysis |
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