Two potato proteins,including a novel RING finger protein (HIP1), interact with the potyviral multifunctional protein HCpro |
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| Authors: | Guo Deyin Spetz Carl Saarma Mart Valkonen Jari P T |
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| Institution: | Institute of Biotechnology, PO Box 56, Viikki Biocenter, FIN-00014 University of Helsinki, Finland. dguo@whu.edu.cn |
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| Abstract: | Potyviral helper-component proteinase (HCpro) is a multifunctional protein exerting its cellular functions in interaction with putative host proteins. In this study, cellular protein partners of the HCpro encoded by Potato virus A (PVA) (genus Potyvirus) were screened in a potato leaf cDNA library using a yeast two-hybrid system. Two cellular proteins were obtained that interact specifically with PVA HCpro in yeast and in the two in vitro binding assays used. Both proteins are encoded by single-copy genes in the potato genome. Analysis of the deduced amino acid sequences revealed that one (HIP1) of the two HCpro interactors is a novel RING finger protein. The sequence of the other protein (HIP2) showed no resemblance to the protein sequences available from databanks and has known biological functions. |
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