Variation of phenotype,ploidy level,and organogenic potential of in vitro regenerated polyploids of <Emphasis Type="Italic">Pyrus communis</Emphasis> |
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Authors: | Qingrong Sun Hongyan Sun Richard L Bell Huifeng Li Li Xin |
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Institution: | (1) Shandong Institute of Pomology, Taian, 27100, Shandong, China;(2) Appalachian Fruit Research Station, USDA, ARS, Kearneysville, WV 25430-2771, USA |
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Abstract: | A wide range of phenotypic variation was observed among neopolyploids obtained from the diploid pear cultivar ‘Fertility’
by in vitro colchicine treatment. The variant plantlets had alterations in leaf characteristics. Neopolyploids had significantly
different ratios of leaf length to leaf width compared to the diploid control. Shoot regeneration from leaf explants and rooting
ability from in vitro shoots of neopolyploids was examined. Regeneration frequencies of shoots from leaf explants of seven
of the nine neopolyploids were significantly decreased compared to the diploid control. The organogenic potential of neopolyploids
was highly genotype-dependent for both shoots and roots. Tetraploid clone 4x − 4 failed to regenerate shoots from leaf explants
and the pentaploid clone 5x − 2 failed to root from in vitro shoots. The results suggest that polyploidization caused the
decrease in or loss of in vitro organogenic potential. Regenerated shoots derived from neopolyploids showed different phenotypes,
depending on the ploidy of the donor plant. |
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Keywords: | |
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