2,4,6-Trinitrophenol degradation by <Emphasis Type="Italic">Bacillus cereus</Emphasis> isolated from a firing range |
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Authors: | Baljinder Singh Jagdeep Kaur Kashmir Singh |
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Institution: | (1) Department of Biotechnology, Panjab University, Chandigarh, 160014, India |
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Abstract: | Bacillus cereus strain PU, isolated from soil contaminated with explosive waste, tolerated up to 1.3 mM 2,4,6 trinitrophenol (TNP) and utilize
it aerobically as sole nitrogen and carbon source. Degradation of TNP was accompanied by stoichiometric release of 2.1 ± 0.15 mol
nitrite/mol TNP at 539 μmol/h g dry cell wt. Metabolism of TNP was accompanied by transient accumulation of an orange-red
metabolite, hydride meisenheimer complex (H-TNP), indicating a metabolic pathway involving complete reductive removal of the
nitro group as nitrite. |
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Keywords: | |
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