Proteomic study and marker protein identification of Caenorhabditis elegans lipid droplets |
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Authors: | Zhang Peng Na Huimin Liu Zhenglong Zhang Shuyan Xue Peng Chen Yong Pu Jing Peng Gong Huang Xun Yang Fuquan Xie Zhensheng Xu Tao Xu Pingyong Ou Guangshuo Zhang Shaobing O Liu Pingsheng |
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Affiliation: | Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China. |
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Abstract: | Lipid droplets (LDs) are a neutral lipid storage organelle that is conserved across almost all species. Many metabolic syndromes are directly linked to the over-storage of neutral lipids in LDs. The study of LDs in Caenorhabditis elegans (C. elegans) has been difficult because of the lack of specific LD marker proteins. Here we report the purification and proteomic analysis of C. elegans lipid droplets for the first time. We identified 306 proteins, 63% of these proteins were previously known to be LD-proteins, suggesting a similarity between mammalian and C. elegans LDs. Using morphological and biochemical analyses, we show that short-chain dehydrogenase, DHS-3 is almost exclusively localized on C. elegans LDs, indicating that it can be used as a LD marker protein in C. elegans. These results will facilitate further mechanistic studies of LDs in this powerful genetic system, C. elegans. |
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