Reduction in vacuolar volume in the tapetal cells coincides with conclusion of the tetrad stage in Arabidopsis thaliana |
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Authors: | Hong Wu Ming Yang |
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Institution: | (1) Department of Botany, Oklahoma State University, Stillwater, OK 74078, USA;(2) Present address: College of Life Sciences South China Agricultural University, Guangzhou, 510642, Guangdong, China |
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Abstract: | Although the tapetum is known for its role in the removal of the tetrad wall, a morphological change in the tapetum correlating
with such a role has not been described. Here we report that in two ecotypes of Arabidopsis thaliana, Landsberg erecta and Columbia, the vacuoles in tapetal cells underwent progressive enlargement prior to the separation of tetrads but became
drastically reduced when tetrads just separated from one another. Such a drastic change in vacuolar volume was not observed
in later anther development. We also observed that the walls of associated tetrads were much less stained with Toluidine Blue
O than the walls of separate tetrads, indicating that the tetrad walls underwent an alteration during the tetrad stage. Furthermore,
we identified the N-terminal propeptide signals for sorting vacuolar proteins in 15 β-1,3-glucanases, five polygalacturonases,
and two endocellulases that are expressed in Arabidopsis young floral buds; all three types of the enzymes are known to participate in degradation of the tetrad wall. These results
suggest that the tapetal vacuoles might be a storage site for these enzymes prior to their secretion to the anther locule. |
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Keywords: | Arabidopsis Callose special wall Glycoside hydrolases Tapetum Tetrad wall Vacuole |
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