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| 番茄环斑病毒单克隆抗体的制备 | |
| 引用本文： | 曹洁,杨翠云,潘卫,于翠,戚中田,代光辉.番茄环斑病毒单克隆抗体的制备[J].微生物学杂志,2007,27(3):35-37. |
| 作者姓名： | 曹洁 杨翠云 潘卫 于翠 戚中田 代光辉 |
| 作者单位： | 1. 第二军医大学,微生物学教研室,上海,200433 2. 上海出入境检验检疫局,上海,200135 3. 上海交通大学,农业与生物学院,上海,201101 |
| 摘 要： | 以纯化的番茄环斑病毒(Tomato ringspot virus,ToRSV)为抗原,注射免疫BALB/c小鼠,将免疫小鼠脾细胞与小鼠骨髓瘤细胞Sp2/0进行融合,经多次细胞筛选及克隆化,获得3株(A8、B7和G9)可分泌抗ToRSV单克隆抗体的杂交瘤细胞株,并以之分别制备小鼠腹水单克隆抗体。经酶联免疫吸附试验检测表明,该3株杂交瘤细胞腹水抗体效价在10-5～10-6之间,且均具有与ToRSV反应的特异性。 |
| 关 键 词： | 番茄环斑病毒 小鼠免疫 单克隆抗体 |
| 文章编号： | 1005-7021(2007)03-0035-03 |
| 修稿时间： | 2006年10月16 |
Preparation of Monoclonal Antibodies against Tomato Ringspot Virus |
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| CAO Jie,YANG Cui-yun,PAN Wei,YU Cui,QI Zhong-tian,DAI Guang-hui.Preparation of Monoclonal Antibodies against Tomato Ringspot Virus[J].Journal of Microbiology,2007,27(3):35-37. | |
| Authors: | CAO Jie YANG Cui-yun PAN Wei YU Cui QI Zhong-tian DAI Guang-hui |
| Abstract: | BALB/c mice were immunized with purified tomato ringspot virus(ToRSV) antigen,and the immunized splenocytes were fused with murine Sp2/0 myeloma cells.Three hybridomas(A8,B7,and G9) capable of secreting monoclonal antibodies(McAbs) against ToRSV were obtained after several screening and subcloning.Ascites McAbs of mice were produced by the three hybridomas respectively.Titers of the Ascites McAbs ranged from 10-3 to 10-4 by ELISA(enzyme-linked immunosorbent assay),and all three McAbs showed specific to ToRSV. |
| Keywords: | tomato ringspot virus immunization of mice monoclonal antibody |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |