Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production |
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Authors: | Roslyn E Wallace Paul J Vasington John C Petricciani Hope E Hopps Douglas E Lorenz Zdenek Kadanka |
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Institution: | (1) Lederle Laboratories Division, American Cyanamid Company, 10965 Pearl River, New York;(2) Bureau of Biologics, Food and Drug Administration, 20852 Rockville, Maryland;(3) Connaught Medical Research Laboratories, University of Toronto, Willowdale, Ontario, Canada |
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Abstract: | Summary The primary goal of this study was to develop and characterize diploid cell lines from fetal tissues of subhuman primates
for use in virus vaccine production. Cell lines have been established from fetal tissues of rhesus and African green monkeys,
and these have been characterized according to the general criteria recommended by the International Cell Committee for Microbiological
Standardization. Of these cell lines, DBS-FRhL-2, developed from lung tissue of a rhesus monkey fetus, has been found to meet
the requirements of populations proposed as substrates for virus vaccines.
Characterization studies show that DBS-FRhL-2 cells have a finite life of more than 50 population doublings in vitro and maintain
the diploid karyotype through an active growth phase. The cells are nontumorigenic, and tests have not revealed the presence
of adventitious agents. They are susceptible to a number of human viruses and can be preserved by freezing without change
in virus susceptibility, cytogenetic, or growth characteristics. These results indicate the need for further evaluation of
this rhesus monkey diploid cell line for acceptability as an alternate substrate in the manufacture of human virus vaccines.
The research upon which this publication is based was performed pursuant to Contract No. NIH-69-100 with the Division of Biologics
Standards of the National Institutes of Health. |
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