Cockroach midgut peptides that regulate cell proliferation, differentiation, and death in vitro |
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Authors: | Takeda M Sakai T Fujisawa Y Narita M Iwabuchi K Loeb M J |
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Institution: | (1) Graduate School of Science and Technology, Kobe University, 657-8501 Kobe, Japan;(2) Suntory Institute for Bioorganic Research, 618-8503 Osaka, Japan;(3) Tokyo University of A&T, 183-8509 Fuchu, Japan;(4) Insect Biocontrol Laboratory, USDA, Beltsville, Maryland |
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Abstract: | Summary The number of insect midgut cells is maintained homeostatically in vivo and in vitro. However, during starvation, the midgut
shrinks and the rate of cell replacement appears to be suppressed. When they undergo metamorphosis, the internal organs of
insects are drastically remodeled by cell proliferation, differentiation, and apoptotic processes, and the net number of cells
usually increases. An extract of 1650 midguts ofPeriplaneta americana was fractionated by highperformance liquid chromatography (HPLC) to obtain the peptides that regulate these processes. The
HPLC fractions were tested for myotropic activity in the foregut and for effects on cell proliferation or loss in primary
cultures of larvalHeliothis virescens midgut cells and in a cell line derived from the last-instar larval fat body ofMamestra brassicae. Some fractions stimulated midgut stem cell proliferation and differentiation, while others caused loss of differentiated
columnar and goblet cells. Other fractions stimulated cell proliferation in the larval fat body cells.
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Keywords: | Periplaneta americana Heliothis virescens Mamestra brassicae peptide growth factor proliferation differentiation cell death |
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