A multigene phylogeny of theGibberella fujikuroi species complex: Detection of additional phylogenetically distinct species |
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Authors: | Kerry O’Donnell Helgard I Nirenberg Takayuki Aoki Elizabeth Cigelnik |
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Institution: | 1. Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, Agricultural University of Athens (AUA), Iera Odos 75, 11855 Athens, Greece;2. Laboratory of Electron Microscopy, Research Centre for Engineering and Agro-food Processing (CREA-IT), Council for Agricultural Research and Economics (CREA), Via Nazionale 38, I-65012 Cepagatti, PE, Italy |
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Abstract: | Phylogenetic relationships within theGibberella fujikuroi species complex were extended to newly discovered strains using nucleotide characters obtained by sequencing polymerase chain
reaction (PCR) amplified DNA from 4 loci used in a previous study nuclear large subunit 28S rDNA, nuclear ribosomal internal
transcribed spacer (ITS) region, mitochondriaal small subunit (mtSSU) ribosomal DNA, and β-tubulin] together with two newly
sampled protein-encoding nuclear genes, translation elongation factor EF-1α and calmodulin. Sequences from the ribosomal ITS
region were analyzed separately and found to contain of two highly divergent, nonorthologous ITS2 types. Phylogenetic analysis
of the individual and combined datasets identified 10 new phylogenetically distinct species distributed among the following
three areas: 2 within Asia and 4 within both Africa and South America. Hypotheses of the monophyly ofFusarium subglutinans and its two formae speciales, f. sp.pini and f. sp.ananas, were strongly rejected by a likelihood analysis. Maximum parsimony results further indicate that the protein-encoding nuclear
genes provide considerably more phylogenetic signal that the ribosomal genes sequenced. Relative apparent synapomorphy analysis
was used to detect long-branch attraction taxa and to obtain a statistical measure of phylogenetic signal in the individual
and combined datasets. |
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Keywords: | biogeography calmodulin DNA sequence elongation factor EF-1α evolution |
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