Endogenous factor in rat liver that converts tyrosine aminotransferase form III to form I: a rapid assay of this converting factor. |
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Authors: | A Boctor A Grossman |
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Institution: | Department of Pharmacology New York University Medical School New York, N. Y. 10016 USA |
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Abstract: | After induction by cortisol, tyrosine aminotransferase (l-tyrosine:2-oxoglutarate aminotransferase, EC 2.6.1.5) present in rat liver homogenates can be resolved into three peaks of activity by CM-Sephadex chromatography. Based on differential elution of these forms by a linear KCl gradient, a three-tube assay was developed that quantitates the amount of form III relative to total enzyme. The assay was used to determine the presence of a factor in the liver that converts tyrosine aminotransferase form III to form I. Definitive evidence for the liberation of such a factor is presented. |
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