Continuous production of chiral 1,3-butanediol using immobilized biocatalysts in a packed bed reactor: promising biocatalysis method with an asymmetric hydrogen-transfer bioreduction |
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Authors: | Email author" target="_blank">Nobuya?ItohEmail author Masatoshi?Nakamura Kousuke?Inoue Yoshihide?Makino |
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Institution: | (1) Department of Biotechnology, Faculty of Engineering (Biotechnology Research Center), Toyama Prefectural University, Kurokawa 5180, Imizu Toyama, 939-0398, Japan |
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Abstract: | An asymmetric hydrogen-transfer biocatalyst consisting of mutated Rhodococcus phenylacetaldehyde reductase (PAR) or Leifsonia alcohol dehydrogenase (LSADH) was applied for some water-soluble ketone substrates. Among them, 4-hydroxy-2-butanone was
reduced to (S)/(R)-1,3-butanediol, a useful intermediate for pharmaceuticals, with a high yield and stereoselectivity. Intact Escherichia coli cells overexpressing mutated PAR (Sar268) or LSADH were directly immobilized with polyethyleneimine or 1,6-diaminehexane
and glutaraldehyde and evaluated in a batch reaction. This system produced (S)-1,3-butanediol 87% enantiomeric excess (e.e.)] with a space time yield (STY) of 12.5 mg h−1 ml−1 catalyst or (R)-1,3-butanediol (99% e.e.) with an STY of 60.3 mg h−1 ml−1 catalyst, respectively. The immobilized cells in a packed bed reactor continuously produced (R)-1,3-butanediol with a yield of 99% (about 49.5 g/l) from 5% (w/v) 4-hydroxy-2-butanoate over 500 h. |
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Keywords: | Chiral 1 3-butanediol Biocatalyst Asymmetric reduction 2-propanol Hydrogen-transfer |
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