Studies on the tissue distribution and stability of "big" CRF (corticotropin-releasing factor). |
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Authors: | N Yasuda M A Greer |
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Institution: | Department of Medicine, Division of Endocrinology University of Oregon Health Sciences Center Portland, OR 97201, USA |
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Abstract: | Extracts of various bovine or rat neural tissues made with 0.1 N HCl, 2N acetic acid or distilled water were fractionated on Sephadex G-100 column with 0.2 N acetic acid as the eluant. A distinct peak of “big” CRF which elutes in the void volume of Sephadex G-100 was observed only with hypothalamic median eminence and hypophyseal stalk. Human serum and extracts of cerebral cortex, neurohypophysis and an ACTH-producing lung tumor, had CRF activity which eluted from Sephadex G-100 with diffuse patterns without a distinct peak. “Big” CRF is stable during storage at ?20 C in water or at 4 C in acid, but progressively disappeared when stored at ?20 C in acid. |
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