A highly efficient preparation of neoglycoconjugate vaccines using subcarriers that bear clustered carbohydrate antigens |
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Authors: | Zhang J Kovác P |
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Institution: | NIDDK, National Institutes of Health, Bethesda, MD 20892-0815, USA. |
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Abstract: | A limited amount of spacer-equipped carbohydrate haptens was linked by reductive amination to a subcarrier, an oligopeptide containing 16 amino groups, to give a hapten-carrying subcarrier (HCS). It was then linked, via the remaining free amino groups, to chicken serum albumin (CSA) to give a cross-linked neoglycoconjugate bearing the haptens in the form of clusters. Alternatively, the same type of a glycoconjugate, but with higher conjugation efficiency, was obtained when HCS was treated successively with squaric acid diethyl ester and CSA. |
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