| piggyBac转座子及其在转基因昆虫中的应用 |
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| 引用本文： | 王建军,;王常春,;韩召军. piggyBac转座子及其在转基因昆虫中的应用[J]. 昆虫知识, 2009, 46(5): 665-672 |
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| 作者姓名： | 王建军, 王常春, 韩召军 |
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| 作者单位： | |
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| 摘 要： | piggyBac是一种从粉纹夜蛾Trichoplusiani.中分离到的、具有TTAA插入位点特异性的DNA转座子。piggyBac可在昆虫基因组中准确切离,转化频率较高,并且不受宿主因子的限制,是目前转基因昆虫研究中应用最广的转座子载体。近年来的研究发现,piggyBac类转座子广泛分布于昆虫和其他生物基因组中。文章从piggyBac的结构、转座特性、在转基因昆虫中的应用以及piggyBac类转座子的分布等几个方面综述了piggyBac的研究进展。
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| 关 键 词： | piggyBac 转座子 转基因昆虫 昆虫基因组 基因载体 |
Advances on piggyBac transposon and its applications in insect transgenesis |
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| Affiliation: | WANG Jian-Jun , WANG Chang- Chun , HAN Zhao-Jun ( 1. College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; 2. Key Laboratory of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture, Nanfing Agricultural University, Nanjing 210095, China) |
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| Abstract: | The piggyBac element is a TTAA-insertion site specific DNA transposon and was originally discovered in Trichoplusia ni. The piggyBac element is capable of precise excision and shows high frequency of transformation in insect genome, and its activity is less restricted by host factors. These characteristics make piggyBac the most widely used gene vector in insect transgenesis. Recent studies revealed that piggyBac-like element is distributed in a wide variety of insect and other organisms. In this review, we introduced the structure and transposition characteristics of piggyBac and its application in insect transgenesis, and the distribution of piggyBac-like elements was also described. |
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| Keywords: | piggyBac piggyBac transposon insect transgenesis insect genome gene vector |
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