Transfer of label from aspartate to malate by the cell-free extract of Sedum mexicanum leaves |
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Authors: | Amagasa, Tadashi Yoshida, Seiichi |
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Affiliation: | Department of Biology, Tokyo Metropolitan University Setagaya-ku, Tokyo 158, Japan |
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Abstract: | The cell-free extract from leaves of Sedum mexicanum, a typicalCAM plant, formed 14C-malate from 14C-aspartate in the presenceof NAD. No reduction of NAD was observed during the reaction.Analysis of this reaction revealed that the transfer of labelfrom l4C-aspartate to malate takes place by the action of malatedehydrogenase and aspartate aminotransferase, and the reactionwas reversible in model experiments with commercial enzymes.Pitfalls in assessing data on dark 14CO2 fixation in CAM arediscussed with reference to the transfer of label between malateand aspartate without actual synthesis. (Received June 2, 1979; ) |
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