| 穴居狼蛛毒中磷酸单酯酶和核酸酶的研究 |
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| 引用本文： | 张雁,刘望夷,吉永华,徐科. 穴居狼蛛毒中磷酸单酯酶和核酸酶的研究[J]. 动物学研究, 1989, 0(2) |
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| 作者姓名： | 张雁 刘望夷 吉永华 徐科 |
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| 作者单位： | 中国科学院上海生物化学研究所，中国科学院上海生物化学研究所，中国科学院上海生理研究所，中国科学院上海生理研究所 上海交通大学生物科学与技术系 |
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| 摘 要： | 本文分别用对硝基酚磷酸酯、3＇-[32p]标记的RNA和大肠杆菌噬菌体入DNA作底物测定了新疆产穴居狼蛛毒中与核酸代谢有关的酶,发现此毒中含有磷酸单酯酶、核糖核酸酶和脱氧核糖核酸酶。
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| 关 键 词： | 穴居狼蛛毒 磷酸单酯酶 核糖核酸酶 脱氧核糖核酸酶 |
STUDY ON PHOSPHOMONOESTERASE,RNase AND DNase IN SPIDER (LYCOSA SINGORIENSIS) VENOM |
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| Zhang Yan Liu Wangyi. STUDY ON PHOSPHOMONOESTERASE,RNase AND DNase IN SPIDER (LYCOSA SINGORIENSIS) VENOM[J]. Zoological Research, 1989, 0(2) |
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| Authors: | Zhang Yan Liu Wangyi |
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| Abstract: | Activities of phosphomonoesterase and nucleases hydrolysing RNA and DNA were presented in a solution of the crude venom from a spider (Lycosa sing-oriensis) collected in Xinjiang Region, China. The substrates for assaying these enzymes were 4-nitrophenyl phosphate disodium salt, 3' terminally 32p-labclled RNA and nonlabelled DNA respectively. After incubation of RNA and DNA with the venom solution, the products were detected by polyacryla-mide gel or agarose gel electrophoresis. |
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| Keywords: | spider venom phosphomonoesterase DNase RNase |
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