An EntD-like phosphopantetheinyl transferase gene from Photobacterium profundum SS9 complements pfa genes of Moritella marina strain MP-1 involved in biosynthesis of docosahexaenoic acid |
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Authors: | Shinji Sugihara Yoshitake Orikasa Hidetoshi Okuyama |
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Affiliation: | (1) Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan;(2) Laboratory of Environmental Molecular Biology, Faculty of Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan |
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Abstract: | The EntD-like phosphopantetheinyl transferase (PPTase) gene, cloned from the eicosapentaenoic acid-producing bacterium Photobacterium profundum strain SS9, has an ORF of 690 bp encoding a 230-amino acid protein. When this PPTase gene was expressed in Escherichia coli with pfaA, pfaB, pfaC and pfaD derived from Moritella marina MP-1, which were four of five essential genes for biosynthesis of docosahexaenoic acid (DHA), the DHA production of the recombinant was 2% (w/w) of total fatty acids. This is the first report showing that the EntD-like PPTase is involved in producing n-3 polyunsaturated fatty acids. Shinji Sugihara and Yoshitake Orikasa contributed equally to this work. |
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Keywords: | Docosahexaenoic acid (DHA) EntD pfaE Phosphopantetheinyl transferase Photobacterium profundum Polyunsaturated fatty acid |
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