Early detection of bean infection by Pseudomonas syringae in asymptomatic leaf areas using chlorophyll fluorescence imaging |
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Authors: | Luis Rodríguez-Moreno Mónica Pineda Julia Soukupová Alberto P. Macho Carmen R. Beuzón Matilde Barón Cayo Ramos |
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Affiliation: | (1) área de Genética, Facultad de Ciencias, Universidad de Málaga, 29071 Malaga, Spain;(2) Department of Biochemistry, Molecular and Cell Biology of Plants, Estación Experimental del Zaidín, CSIC, 18008 Granada, Spain;(3) Institute of Systems Biology and Ecology, Academy of Sciences of Czech Republic, Zámek 136, Nove Hrady, 37333, Czech Republic;(4) Institute of Physical Biology, University of South Bohemia, Zámek 136, Nove Hrady, 37333, Czech Republic |
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Abstract: | Chlorophyll fluorescence imaging has been used to analyse the response elicited in Phaseolus vulgaris after inoculation with Pseudomonas syringae pv. phaseolicola 1448A (compatible interaction) and P. syringae pv. tomato DC3000 (incompatible interaction). With the aim of modulating timing of symptom development, different cell densities were used to inoculate bean plants and the population dynamics of both bacterial strains was followed within the leaf tissue. Fluorescence quenching analysis was carried out and images of the different chlorophyll fluorescence parameters were obtained for infected as well as control plants at different timepoints post-infection. Among the different parameters analysed, we observed that non-photochemical quenching maximised the differences between the compatible and the incompatible interaction before the appearance of visual symptom. A decrease in non-photochemical quenching, evident in both infiltrated and non-infiltrated leaf areas, was observed in P. syringae pv. phaseolicola-infected plants as compared with corresponding values from controls and P. syringae pv. tomato-infected plants. No photoinhibitory damage was detected, as the maximum photosystem II quantum yield remained stable during the infection period analysed. |
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Keywords: | Chlorophyll fluorescence imaging Hypersensitive response Phaseolus vulgaris Pseudomonas syringae |
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