Structure and mechanism of the diterpene cyclase ent-copalyl diphosphate synthase |
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Authors: | Köksal Mustafa Hu Huayou Coates Robert M Peters Reuben J Christianson David W |
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Institution: | Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania, USA. |
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Abstract: | The structure of ent-copalyl diphosphate synthase reveals three α-helical domains (α, β and γ), as also observed in the related diterpene cyclase taxadiene synthase. However, active sites are located at the interface of the βγ domains in ent-copalyl diphosphate synthase but exclusively in the α domain of taxadiene synthase. Modular domain architecture in plant diterpene cyclases enables the evolution of alternative active sites and chemical strategies for catalyzing isoprenoid cyclization reactions. |
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