A Study on Erythrocyte Membrane Plasmalogen in Myotonic Dystrophy |
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Authors: | Yasunobu Antoku Tetsuo Sakai Kohsuke Tsukamoto Ikuo Goto Hiroshi Iwashita Yoshigoro Kuroiwa |
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Affiliation: | Department of Neurology, National Chikugo Hospital, Chikugo City;Department of Neurology, Neurological Institute, Faculty of Medicine, Kyushu University, Higashi-ku, Fukuoka, Japan1 |
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Abstract: | Phospholipid classes that included plasmalogens of erythrocyte membranes in seven myotonic dystrophy (MyD) patients and seven normal controls were analyzed by HPLC. No significant difference in phospholipid classes was found between patients with MyD and normal controls, but there was a visible difference in peak profiles of compounds of the phosphatidylethanolamine class. In the study of plasmalogens, we used two preparation methods: exposure to HCl and deacylation with mild alkaline. The area ratio of the plasmalogen form to the diacyl form in the phosphatidylethanolamine class of MyD erythrocyte membranes was significantly lower than that of normal controls. Fatty acid analyses showed that fatty acids of both phosphatidylethanolamine subclasses have less unsaturation in MyD. |
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Keywords: | Myotonic dystrophy Erythrocyte membrane plasmalogen HPLC |
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