Effect of transformation ofAzotobacter vinelandii with the low copy number plasmid pRK290 |
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Authors: | Dr Bernard R Glick Barbara J Butler Colin I Mayfield J J Pasternak |
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Institution: | (1) Department of Biology, University of Waterloo, N2L3G1 Waterloo, Ontario, Canada |
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Abstract: | We previously observed that whenAzotobacter vinelandii was transformed by different broad-host-range plasmids, normal cellular functions such as growth and siderophore production are impaired. In the present work, whenA. vinelandii was transformed with the low copy number plasmid pRK290, the extent of this metabolic impairment was lessened, as evidenced by increased siderophore production and moderate levels of growth on medium that lacks added iron. It is concluded that the severity of the plasmid-induced metabolic load reflects the relative level of expression of plasmid-encoded proteins. |
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