| 一株杀虫抗生素产生菌的筛选、鉴定及活性物质稳定性研究 |
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| 引用本文： | 李涛,覃拥灵,陈桂光,李楠,张云开,梁智群.一株杀虫抗生素产生菌的筛选、鉴定及活性物质稳定性研究[J].中国生物工程杂志,2007,27(4):54-58. |
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| 作者姓名： | 李涛 覃拥灵 陈桂光 李楠 张云开 梁智群 |
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| 作者单位： | 广西大学生命科学与技术学院 广西大学生命科学与技术学院 广西大学生命科学与技术学院 广西大学生命科学与技术学院 广西大学生命科学与技术学院 广西大学生命科学与技术学院 |
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| 摘 要： | 通过卤虫初步筛选及家蚕复筛，从土壤中分离得到一株产高杀虫活性物质菌株 GX-29。经16S rDNA基因序列分析表明该菌为极暗黄链霉菌。利用卤虫活性追踪对其活性物质稳定性进行初步研究，结果表明温度、紫外线对其杀虫活性影响不显著；中性及偏碱性条件下，其杀虫活性稳定。
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| 关 键 词： | 杀虫活性 卤虫 筛选模型 稳定性 |
| 收稿时间： | 2006-10-24 |
| 修稿时间： | 2006年10月23 |
Screen and Identification of Insecticidal Antibiotics Producing Strain and Studies on the Stability of Bioactive Substances |
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| LI Tao,QIN Yong-ling,CHEN Gui-guang,LI Nan,ZHANG Yun-kai,LIANG Zhi-qun.Screen and Identification of Insecticidal Antibiotics Producing Strain and Studies on the Stability of Bioactive Substances[J].China Biotechnology,2007,27(4):54-58. |
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| Authors: | LI Tao QIN Yong-ling CHEN Gui-guang LI Nan ZHANG Yun-kai LIANG Zhi-qun |
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| Abstract: | By using Brine Shrimp as primary screening assay and silkworm as second screening assay, the strain of GX-29 which with high insecticidal activity substances were selected from the soil. It was identified as Streptomyces fulvissimus based on physiological and biochemical characteristics and 16S rDNA sequence analysis. The insecticidal stability was also studied by means of Brine Shrimp tracking activity. The result shows that temperature, ultraviolet are not greatly affected the insecticidal activity, and its bioactivity kept steady in neutral and slight alkaline condition. |
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| Keywords: | Insecticidal activity Screening assay Identification Stability |
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