The mechanisms by which vasoactive intestinal peptide (VIP) and thyrotropin releasing hormone (TRH) stimulate prolactin release from pituitary cells |
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Authors: | Trine Bjøro Olav Sand Bjørn Chr. Østberg Jan O. Gordeladze Peter Torjesen Kaare M. Gautvik Egil Haug |
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Affiliation: | (1) Hormone Laboratory, Aker Hospital, N-0514 Oslo, Norway;(2) Department of Biology, University of Oslo, N-0316 Oslo, Norway;(3) Institute of Medical Biochemistry, University of Oslo, N-0316 Oslo, Norway |
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Abstract: | The effect of vasoactive intestinal peptide (VIP) on prolactin (PRL) secretion from pituitary cells is reviewed and compared to the effect of thyrotropin releasing hormone (TRH). These two peptides induced different secretion profiles from parafused lactotrophs in culture. TRH was found to increase PRL secretion within 4 s and induced a biphasic secretion pattern, while VIP induced a monophasic secretion pattern after a lag time of 45–60 s.The secretion profiles are compared to changes in adenylate cyclase activity, production of inositol polyphosphates, changes in intracellular calcium concentrations and changes in electrophysiological properties of the cell membrane.Abbreviations AC adenylate cyclase - DG diacyglycerol - GH growth hormone - GTP guanosine trisphosphate - Gi GTP binding proteins that mediate inhibition of adenylate cyclase and that are pertussis toxin sensitive - Gs GTP binding protein that mediates stimulation of adenylate cyclase - GH cells clonal rat pituitary tumor cells producing PRL and/or growth hormone - GH3 GH4C1 and GH4B6 subclones of GH cells - PKA protein kinase A - PKC protein kinase C - PLC phospholipase C - PRL prolactin - TPA 12-O-tetradecanoyl phorbol 13-acetate - TRH thyrotropin releasing hormone - VIP vasoactive intestinal peptide |
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Keywords: | vasoactive intestinal peptide (VIP) thyrotropin releasing hormone (TRH) prolactin (PRL) lactotrophs anterior pituitary second messengers hormone secretion adenylate cyclase calcium inositol trisphosphate |
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