Purification and characterization of the gag gene products of avian-type C retroviruses by high-pressure liquid chromatography |
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| Authors: | A S Bhown J C Bennett J E Mole E Hunter |
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| Institution: | Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Szeged 6701 P.O. Box 521, Hungary |
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| Abstract: | We have developed an inexpensive procedure for bacteriophage purification suitable for small- and medium-scale preparations (up to one liter of lysate). The method consists of precipitation with polyethylene glycol 6000 and gel chromatography on a Bio-Gel A-5m column. The purity of the phage preparation is comparable to that obtained by CsCl step gradient ultracentrifugation. |
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