The effect of protease on stability of cellulase and xylanase fromCellulomonas flavigena

Summary Crude preparations of extracellular cellulase and xylanase fromCellulomonas flavigena at 4°C show a rapid loss of activity. With the protease inhibitors aprotinin and -2 macroglobulin this loss of activity could be dramatically reduced. Cellulase and xylanase extracted from a protease negative mutant were also more stable. When the cellulase and xylanase was purified by DEAE sepharose from wild type strains, the protease activity could be separated, such preparations of cellulase and xylanase were extremely stable.