Isolation and characterization of a 5S RNA-protein complex from human placental ribosomes |
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| Authors: | B Peeters L Vanduffel W Rombauts |
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| Institution: | 1. Afdeling Biochemie, Department Humane Biologie, Fakulteit Geneeskunde, Katholieke Universiteit Leuven, B-300, Leuven, Belgium
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| Abstract: | Large ribosomal subunits treated with EDTA change their sedimentation rate in sucrose gradients and lose the 5S RNA molecule, which is released in a ribonucleoprotein particle sedimenting at about 7S. The proteins bound in this complex were identified by two-dimensional (2-D) polyacrylamide gel electrophoresis as ribosomal proteins L3 and L4, both having a molecular weight of about 37000. |
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