Pax3( GFP ) , a new reporter for the melanocyte lineage, highlights novel aspects of PAX3 expression in the skin |
| |
Authors: | Djian-Zaouche Johanna Campagne Cécile Reyes-Gomez Edouard Gadin-Czerw Stéphanie Bernex Florence Louise Anne Relaix Frédéric Buckingham Margaret Panthier Jean-Jacques Aubin-Houzelstein Geneviève |
| |
Institution: | 1. INRA, UMR955?Génétique Fonctionnelle et Médicale, Ecole Nationale Vétérinaire d’Alfort, Maisons‐Alfort, France;2. Université Paris‐Est, Ecole Nationale Vétérinaire d’Alfort, UMR955?Génétique Fonctionnelle et Médicale, Maisons‐Alfort, France;3. Université Paris‐Est, Ecole Nationale Vétérinaire d’Alfort, Unité d’Embryologie, d’Histologie et d’Anatomie Pathologique, Maisons‐Alfort, France;4. Plate‐forme de Cytométrie, Département d’Immunologie, Institut Pasteur, Paris, France;5. CNRS URA 2578, Département de Biologie du Développement;6. Institut Pasteur, Unité de Génétique Moléculaire du Développement, Paris, France;7. CNRS URA 2578, Département de Biologie du Développement;8. USC INRA 2026, Institut Pasteur, Unité de Génétique Fonctionnelle de la Souris, Paris, France |
| |
Abstract: | The paired box gene 3 (Pax3) is expressed during pigment cell development. We tested whether the targeted allele Pax3(GFP) can be used as a reporter gene for pigment cells in the mouse. We found that enhanced green fluorescent protein (GFP) can be seen readily in every melanoblast and melanocyte in the epidermis and hair follicles of Pax3(GFP/+) heterozygotes. The GFP was detected at all differentiation stages, including melanocyte stem cells. In the dermis, Schwann cells and nestin-positive cells of the piloneural collars resembling the nestin-positive hair follicle multipotent stem cells exhibited a weaker GFP signal. Pigment cells could be purified by fluorescent activated cell sorting and grown in vitro without feeder cells, giving pure cultures of melanocytes. The Schwann cells and nestin-positive cells of the piloneural collars were FACS-isolated based on their weak expression of GFP. Thus Pax3(GFP) can discriminate distinct populations of cells in the skin. |
| |
Keywords: | mouse paired‐box gene Dct::lacZ nestin‐positive cells melanoblast melanocyte stem cell |
本文献已被 PubMed 等数据库收录! |
|