A novel universal linker for efficient synthesis of phosphorothioate oligonucleotides |
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Authors: | Wang Zhiwei Olsen Phil Ravikumar Vasulinga T |
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Affiliation: | Isis Pharmaceuticals, Carlsbad, California 92008. USA. |
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Abstract: | A versatile and conformationally preorganized universal linker molecule is reported here for efficient synthesis of phosphorothioate oligonucleotides. With respect to nucleoside loaded support, comparable yield and quality based on ion-pair LC-MS are obtained for both deoxy and 2'-O-methoxyethyl modified phosphorothioate oligonucleotides. No 3'-phosphate or phosphorothioate monoester or any modification of universal molecule still attached to oligonucleotide was observed. [structure: see text] |
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