Repetitive, genome-specific probes in wheat (Triticum aestivum L. em Thell) amplified with minisatellite core sequences |
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| Authors: | D J Somers Z Zhou P J Bebeli J P Gustafson |
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| Institution: | (1) Plant Science Unit, Department of Agronomy, University of Missouri, 65211 Columbia, MO, USA;(2) United States Department of Agriculture-Agricultural Research Service, Plant Genetics Research Unit, University of Missouri, 65211 Columbia, MO, USA;(3) Department of Plant Breeding and Biometry, Athens Agricultural University, Athens, Greece;(4) Present address: Agriculture and Agri-Food Canada, Research Centre, 107 Science place Saskatoon, S7N-OX2, SK, Canada;(5) Present address: Department of Botany and Plant Sciences, University of California, 92521 Riverside, CA, USA |
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| Abstract: | The detection and analysis of DNA polymorphisms in crops is an essential component of marker-assisted selection and cultivar identification in plant breeding. We have explored the direct amplification of minisatellite DNA by PCR (DAMD-PCR) as a means for generating DNA probes that are useful for detecting DNA polymorphisms and DNA fingerprinting in wheat. This technique was facilitated by high-stringency PCR with known plant and animal minisatellite core sequences as primers on wheat genomic DNA. The products of DAMD-PCR from Triticum aestivum, T. durum, T. monococcum, T. speltoides and T. tauschii showed a high degree of polymorphism and the various genomes could be identified. Cloning of the DAMD-PCR products and subsequent Southern hybridization frequently revealed polymorphic probes showing a good degree of genome specificity. In addition, polymorphic, single locus, and moderately dispersed PCR products were cloned that may have a potential for DNA fingerprinting. Our experiments were limited primarily to diploid wheats and the results indicated that DAMD-PCR may isolate genome-specific probes from wild diploid wheat species that could be used to monitor genome introgression into hexaploid wheat.This paper reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation for its use by the USDA or the University of Missouri. Contribution from the University of Missouri, the Agricultural Experimental Station and U.S. Department of Agriculture-Agricultural Research Service, Plant Genetics Research Unit, journal series No. 12523 |
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| Keywords: | Genome-specific DAMD Minisatellite PCR Triticum Wheat |
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