On-line monitoring of oxygen in Tubespin, a novel, small-scale disposable bioreactor |
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Authors: | Kui Xie Xiao-Wei Zhang Li Huang Yi-Ting Wang Yun Lei Jing Rong Chui-Wen Qian Qiu-Ling Xie Yi-Fei Wang An Hong Sheng Xiong |
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Affiliation: | Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, Guangzhou, 510632, Guangdong, People's Republic of China. |
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Abstract: | A novel, optical sensor was fixed in a new type of disposable bioreactor, Tubespin, for the on-line (real-time) monitoring of dissolved oxygen concentrations during cell culture. The cell density, viability and volumetric mass transfer coefficient were also determined to further characterize the bioreactors. The kLa value of the Tubespin at standard conditions was 24.3 h−1, while that of a spinner flask was only 2.7 h−1. The maximum cell density in the Tubespin bioreactor reached 6 × 106 cells mL−1, which was two times higher than the cell density in a spinner flask. Furthermore, the dynamic dissolved oxygen level was maintained above 90% air-saturation in the Tubespin, while the value was only 1.9% in a spinner flask. These results demonstrate the competitive advantage of using the Tubespin system over spinner flasks for process optimization and scale-down studies of oxygen transfer and cell growth. |
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Keywords: | Tubespin Spinner flask Dissolved oxygen kLa |
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