‘章姬'草莓茎段愈伤组织诱导及高频植株再生体系的建立北大核心CSCD

In order to cultivate an improved variety with higher potential yield and establish an efficient in vitro regeneration system of strawberry ‘Akihim'(Fragaria × ananassa), callus induction and plant regeneration protocol was designed for solve browning problem. A formal L9(34)orthogonal experiment was designed to investigate the browning research in primary culture using explants of creeping stems excised from aseptic seedlings. Based on the improved medium above, single-factor experiments were conducted to select effective plant growth regulators and appropriate concentrations on blank MS medium. A L9(34)orthogonal experiment was designed to study effects of types and concentrations of plant growth regulators on callus induction, adventitious shoot formation, and plant regeneration. The results indicated that MS medium was inferior to B5 and 1/2MS in inhibiting browning condition. The browning rate was dramatically reduced while the callus still survived on the medium with the addition of 20 g·L-1 Na2S2O3. The callus induction and shoot formation of the explants were observed on MS + 0.1 mg· L-1 6-BA + 0.05 mg·L-1 2, 4-D + 0.1 mg·L-1 NAA with effective inhibiting browning. The optimal medium protocol for multiple shoots proliferation was MS + 0.1 mg·L-1 6-BA + 0.1 mg·L-1 NAA where the proliferation coefficient was 12.86 after 30 d. Healthily regenerated plants were yielded on culture medium 1/2MS + 1.0 g·L-1 AC after 35 d, with a rooting rate of 92.50%. More than 95% of plantlets survived after transplanting into field. The rapid propagation system is helpful to provide homogeneous progeny and high quality seedlings for cultivation of strawberry ‘Akihime' as well as a technical reference for other strawberry species in vitro regeneration. ABSTRACT FROM AUTHOR]