An analysis of the mechanism by which cetiedil inhibits sickling |
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Authors: | Abu-Salah Khalid M Gambo Al-Hafiz A |
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Institution: | Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia. |
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Abstract: | A study has been carried out into the effects of cetiedil on the activities of Na+, K+ and Ca2+, Mg2+-ATPases of the normal human erythrocyte membrane. In general, cetiedil inhibits both ATPases activities but with characteristic inhibition profiles and varying degrees of efficacy. The activities were inhibited non-competitively at the cetiedil concentration which caused 50% inhibition of each enzyme. In addition, the effects of cetiedil on the transport of K+ and phosphate ions across the membrane were monitored and compared. Cetiedil was found to stimulate K+ release and to inhibit phosphate uptake. At low concentrations, both processes were concentration dependent. Stimulation of K+ efflux reached a plateau at a concentration of 1.2 mM. The antisickling effect of cetiedil is explained mainly in the light of the changes it induces in the activities of membrane-bound ATPases and the permeability properties of the erythrocyte membrane to cations and anions. |
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Keywords: | Erythrocytes ATPases K+-efflux Phosphate transport Cetiedil Sickling |
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