Determination of metabolites by observing the change in reaction rate during the early course of a first order reaction, as applied to fructose-1 phosphate. |
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| Authors: | K Sahlin R C Harris E Hultman |
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| Affiliation: | 1. Dept. of Metabolic Research, S:t Eriks sjukhus, Stockholm, Sweden;2. Dept. of Clinical Chemistry, Beckomberga sjukhus, Bromma, Sweden |
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| Abstract: | An enzymatic method is described for the determination of fructose-1-phosphate photometrically with fructose-6-phosphate kinase. By using the reaction rate to calculate the absorbance at the end of the reaction, the precision of the method is improved and the time taken for one assay reduced from 2 to 3 hr to about 1 hr. |
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