Coordination of Mn++ ions at contact sites between tRNA and aminoacyl-tRNA synthetase. |
| |
| Authors: | J M Backer S V Vocel L M Weiner S I Oshevskii O I Lavrik |
| |
| Institution: | 1. Institute of Chemical Kinetics and Combustion of the Siberian Division of the Academy of Sciences of the USSR USSR;2. Institute of Organic Chemistry of the Siberian Division of the Academy of Sciences of the USSR Novosibirsk 90, USSR |
| |
| Abstract: | By means of PMR and ESR study the shielding of Mn++ ions by aminoacyl-tRNA synthetase has been detected in the aminoacyl-tRNA synthetase - tRNA complex at pH 7.5. At pH 6 this effect was not observed. We propose that ions interact with certain aminoacyl-tRNA synthetase groups protonated when passing to slightly acid pH. The role of Mn++ and Mg++ ions in the formation of a functionally active complex tRNA-aminoacyl-tRNA synthetase is discussed. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
