Identification of bifidobacteria isolated from Asian elephant (Elephas maximus) |
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Authors: | Vera Bunesova Eva Vlkova Vojtech Rada Jiri Killer Vladimir Kmet |
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Institution: | 1. Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycká 129, Prague 6-Suchdol, 1652 1, Czech Republic 3. Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 4-Kr?, 14200, Czech Republic 2. Institute of Animal Physiology of Slovak Academy of Sciences, Soltesovej 4, Kosice, 040 01, Slovakia
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Abstract: | Bifidobacteria are considered as one of the key genera in intestinal tracts of animals, and their species composition vary depending on the host. The aim of this study was to identify faecal bifidobacteria from Asian elephants (Elephas maximus), housed in Zoological gardens (Ostrava, Czech Republic). Using culturing, bifidobacteria were found in counts 7.60?±?0.56 log CFU/g. Twenty-six pure strains were isolated from faeces of Asian elephant. The isolates were clustered into two groups according to fingerprinting profiles and fermentation characteristic. Bacteria were identified by a combination of MALDI-TOF MS, PCR methods and sequencing as B. boum (12 isolates) and B. adolescentis (14 isolates). Elephant strains showed different fingerprinting profiles than type and collection strains. Since these two species are frequently isolated from gastrointestinal tract of herbivores, they seem to be typical of animals fed plant diets. |
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