Hairy roots induced by Agrobacterium rhizogenes and production of regenerative plants in hairy root cultures in maize |
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Authors: | Hongwei Xu Xiaofu Zhou Jingmei Lu Junjie Wang Xingzhi Wang |
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Affiliation: | (1) School of Life Sciences, Northeast Normal University, Changchun, 130024, China;(2) Institute of Gene Engineering, Jilin Normal University, Siping, 136000, China |
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Abstract: | Hairy roots of maize were induced by infecting 15-d calli with Agrobacterium rhizogenes. The hairy roots cultured in hormone-free media showed the vigorous growth and typical hairy root features. The regenerated plants were produced from hairy roots in MS media supplemented with 1.6 mg/L ZT and 0.4 mg/L NAA. The PCR-Southern hybridization demonstrated that T-DNA had been integrated into the chromosome of regenerated plants. These authors contributed equally to this work. |
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Keywords: | Agrobacterium rhizogenes maize hairy root PCR-Southern |
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