| 黑木耳漆酶纯化的研究 |
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| 引用本文： | 张介驰,韩增华,张丕奇,戴肖东,孔祥辉,马庆芳.黑木耳漆酶纯化的研究[J].生物技术,2007,17(2):55-57. |
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| 作者姓名： | 张介驰 韩增华 张丕奇 戴肖东 孔祥辉 马庆芳 |
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| 作者单位： | 黑龙江省科学院应用微生物研究所,黑龙江,哈尔滨,150010 |
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| 基金项目： | 黑龙江省杰出青年科学基金 |
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| 摘 要： | 目的:研究黑木耳(Auricularia auricula)“黑29”的漆酶纯化,为进一步酶学性质的开展、酶应用和酶基因克隆提供理论基础。方法:采用硫酸铵分级沉淀和柱层析技术分离蛋白,通过PAGE和SDS-PAGE电泳检测蛋白。结果:SDS-PAGE电泳检测发现粗酶液含三种漆酶,分子量大小分别为LacA(60kD)、LacB(34kD)、LacC(19kD);纯化后获得纯化的单一漆酶LacA、LacC组分。结论:得漆酶两个单一组分,为进一步漆酶研究奠定基础。
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| 关 键 词： | 黑木耳 漆酶 同工酶 纯化 |
| 文章编号： | 1004-311X（2007）02-0055-03 |
| 收稿时间： | 2007-02-03 |
| 修稿时间： | 2007-03-12 |
Purification of Laccase by Auricularia auricula |
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| ZHANG Jie-chi,HAN Zeng-hua,ZHANG Pi-qi,DAI Xiao-dong,KONG Xiang-hui,MA Qing-fang.Purification of Laccase by Auricularia auricula[J].Biotechnology,2007,17(2):55-57. |
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| Authors: | ZHANG Jie-chi HAN Zeng-hua ZHANG Pi-qi DAI Xiao-dong KONG Xiang-hui MA Qing-fang |
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| Institution: | Institue of Applied Microbiology, Heilongjiang Academy of Scienec, Harbin 150010, China |
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| Abstract: | Objective:In order to study enzymatic properties and enzymatic gene colong,purification of laccase by Auricularia auricula was studied in the paper.Methods:The laccase from Auricularia auricula "Hei 29" was purified by two steps including treatment of(NH4)2SO4 and chromatography on DEAE-Sephacel.Results:PAGE and SDS-PAGE for laccase crude extract showed that three kinds of isoenzymes exist in Hei 29.The molecular weight of the three isoenzymes was determined by SDS-PAGE: LacA(69kD),LacB(34kD),LacC(19kD).The LacA and LacC were purified by ammonium sulphate fractionation,DEAE-Sephacel chromatography.Conclusion:The study gained two purifications: LacA and LacC. |
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| Keywords: | Auricularia auricular laccase isoenzymes crude extract |
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