A plant transformation vector with a minimal T-DNA |
| |
| Authors: | Klaus Düring |
| |
| Institution: | (1) Center for Applied Plant Molecular Biology, University of Hamburg, Ohnhorststr. 18, D-22609 Hamburg, Germany;(2) Present address: Institute for Breeding Methods in Vegetables, Federal Centre for Breeding Research on Cultivated Plants, Neuer Weg 22/23, D-06484 Quedlinburg, Germany |
| |
| Abstract: | Plant transformation, viaAgrobacterium tumefaciens, is usually performed with binary vectors. Most of the available binary vectors contain within the T-DNA (which is transferred to the plant genome) components not required for the intended modification. These additional sequences may cause potential risks during field testing of the transgenic plants or even more in the case of commercialization. The aim of this study was to produce a plant transformation vector which only contains a selectable and screenable marker gene and a multiple cloning site for insertion of promoter::foreign gene::terminator cassettes from other plasmids. |
| |
| Keywords: | transformation vector Agrobacterium tumefaciens plant transformation T-DNA nptII gene |
| 本文献已被 SpringerLink 等数据库收录！ |
|
