pHsh vectors,a novel expression system of <Emphasis Type="Italic">Escherichia coli</Emphasis> for the large-scale production of recombinant enzymes |
| |
| Authors: | Huawei Wu Jianjun Pei Yu Jiang Xin Song Weilan Shao |
| |
| Institution: | (1) Jiangsu Key Laboratory for Biodiversity and Bioresources, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210046, People’s Republic of China;(2) College of Life Sciences, Yangtze University, Jingzhou, 434023, People’s Republic of China;(3) Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, People’s Republic of China; |
| |
| Abstract: | Gene expression system Hsh is developed to increase enzyme production and to decrease the cost in the induction of gene expression
in Escherichia coli. The vectors of Hsh system were constructed by combining a synthesized heat-shock promoter with a synthesized terminator
and an origin of replication derived from pUC19 in which the expression of foreign genes was regulated by an alternative sigma
factor, σ32 of E. coli. In comparison, the Hsh promoter gave a 2.4-fold higher production for xynIII gene encoding a xylanase than existing heat-shock inducible promoter p
L, 1.2-fold and 3-fold production for xar gene encoding a arabinosidase than trc and T7 promoter, respectively. The flow-in-heat technique created a rapid rise in temperature for effective induction of gene expression
in bioreactor scale. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
